HCC827-CAS9 cell line

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HCC827-CAS9 Order

HCC827-CAS9 cell line
Catalog#
YC-C043-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
HCC827-CAS9 cell line
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Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
HCC827-CAS9
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the HCC827 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HCC827-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
HCC827-CAS9 Cas9 15.03288746 2.38374043
HCC827-CAS9 β-actin 12.64914703
HCC827 Cas9 35.32550430 22.70522594
HCC827 β-actin 12.62027836
Cutting Efficiency Validation
HCC827-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the HCC827-CAS9 stable cell pool which is electroporated by METTL5 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HCC827		 STR Info (Cell bank)
Cell Line:HCC-827
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 9999
D7S820 11121112
D16S539 12121212
VWA 18181818
TH01 6666
AMEL XXXX
TPOX 8888
CSF1PO 11111111
D12S391 1717
FGA 2424
D2S1338 172024.0
D21S11 3131
D18S51 1313
D8S1179 1212
D3S1358 1717
D6S1043 1212
PENTAE 2020
D19S433 1414
PENTAD 1414
D1S1656 121718.0
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
HCC827-CAS9 cell line
HCC827-CAS9
Catalog#
YC-C043-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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